Differential CARS microscopy with linearly chirped femtosecond laser pulses

Wolfgang Langbein; Israel Rocha-Mendoza; Francesco Masia; Claudia Di Napoli; Iestyn Pope; Peter Watson; Paola Borri

doi:10.1117/12.873872

11 February 2011 Differential CARS microscopy with linearly chirped femtosecond laser pulses

Wolfgang Langbein, Israel Rocha-Mendoza, Francesco Masia, Claudia Di Napoli, Iestyn Pope, Peter Watson, Paola Borri

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79031I (2011) https://doi.org/10.1117/12.873872
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We demonstrate frequency differential CARS (D-CARS) using femtosecond laser pulses linearly chirped by glass elements of high group-velocity dispersion. By replicating the Pump-Stokes pair into a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference by glass dispersion, we adjust the Raman frequency probed by each pair in an intrinsically stable way. The resulting CARS intensities are detected simultaneously by a single photomultiplier as sum and difference using lock-in detection. We demonstrate imaging of living cells with strongly suppressed non-resonant background. We also show D-CARS using a single femtosecond laser source.

Citation Download Citation

Wolfgang Langbein, Israel Rocha-Mendoza, Francesco Masia, Claudia Di Napoli, Iestyn Pope, Peter Watson, and Paola Borri "Differential CARS microscopy with linearly chirped femtosecond laser pulses", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031I (11 February 2011); https://doi.org/10.1117/12.873872

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available