11 February 2011 Nanosurgery with near-infrared 12-femtosecond and picosecond laser pulses
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Laser-assisted surgery based on multiphoton absorption of NIR laser light has great potential for high precision surgery at various depths within the cells and tissues. Clinical applications include refractive surgery (fs-LASIK). The non-contact laser method also supports contamination-free cell nanosurgery. Here we apply femtosecond laser scanning microscopes for sub-100 nm surgery of human cells and metaphase chromosomes. A mode-locked 85 MHz Ti:Sapphire laser with an M-shaped ultrabroad band spectrum (maxima: 770 nm/830 nm) with an in situ pulse duration at the target ranging from 12 femtoseconds up to 3 picoseconds was employed. The effects of laser nanoprocessing in cells and chromosomes have been quantified by atomic force microscopy (AFM) and electron microscopy. These studies demonstrate the potential of extreme ultrashort femtosecond laser pulses at low mean milliwatt powers for sub-100 nm surgery.
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Aisada Uchugonova, Aisada Uchugonova, Huijing Zhang, Huijing Zhang, Cornelius Lemke, Cornelius Lemke, Karsten König, Karsten König, } "Nanosurgery with near-infrared 12-femtosecond and picosecond laser pulses", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031N (11 February 2011); doi: 10.1117/12.875032; https://doi.org/10.1117/12.875032

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