Wide-field two-photon microscopy with temporal focusing and HiLo background rejection

Elijah Y. S. Yew; Heejin Choi; Daekeun Kim; Peter T. C. So

doi:10.1117/12.876068

28 February 2011 Wide-field two-photon microscopy with temporal focusing and HiLo background rejection

Elijah Y. S. Yew; Heejin Choi; Daekeun Kim; Peter T. C. So

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79031O (2011) https://doi.org/10.1117/12.876068
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Scanningless depth-resolved microscopy is achieved through spatial-temporal focusing and has been demonstrated previously. The advantage of this method is that a large area may be imaged without scanning resulting in higher throughput of the imaging system. Because it is a widefield technique, the optical sectioning effect is considerably poorer than with conventional spatial focusing two-photon microscopy. Here we propose wide-field two-photon microscopy based on spatio-temporal focusing and employing background rejection based on the HiLo microscope principle. We demonstrate the effects of applying HiLo microscopy to widefield temporally focused two-photon microscopy.

Citation Download Citation

Elijah Y. S. Yew, Elijah Y. S. Yew, Heejin Choi, Heejin Choi, Daekeun Kim, Daekeun Kim, Peter T. C. So, Peter T. C. So, } "Wide-field two-photon microscopy with temporal focusing and HiLo background rejection", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031O (28 February 2011); doi: 10.1117/12.876068; https://doi.org/10.1117/12.876068

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