A multispectral FLIM tomograph for in-vivo imaging of skin cancer

C. B. Talbot; R. Patalay; I. Munro; H. G. Breunig; K. König; Y. Alexandrov; S. Warren; A. Chu; G. W. Stamp; M. A. A. Neil; Paul M. W. French; Christopher Dunsby

doi:10.1117/12.873567

28 February 2011 A multispectral FLIM tomograph for in-vivo imaging of skin cancer

C. B. Talbot, R. Patalay, I. Munro, H. G. Breunig, K. König, Y. Alexandrov, S. Warren, A. Chu, G. W. Stamp, M. A. A. Neil, Paul M. W. French, Christopher Dunsby

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79032B (2011) https://doi.org/10.1117/12.873567
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

To aid the in vivo diagnosis of skin lesions, we present the design and implementation of a 4 channel FLIM detector and a hyperspectral imaging detector into a clinically licensed commercial two-photon tomograph. We have also implemented image segmentation algorithms to facilitate the automated processing of the large volumes of data produced. The first detector is based on multispectral time correlated single photon counting, providing four channel fluorescence lifetime images. The second detector is a prism-based CCD hyperspectral imager. These detectors provide the capability to extract the relative content and state of autofluorescence compounds present in biological tissue.

Citation Download Citation

C. B. Talbot, R. Patalay, I. Munro, H. G. Breunig, K. König, Y. Alexandrov, S. Warren, A. Chu, G. W. Stamp, M. A. A. Neil, Paul M. W. French, and Christopher Dunsby "A multispectral FLIM tomograph for in-vivo imaging of skin cancer", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032B (28 February 2011); https://doi.org/10.1117/12.873567

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available