Raman spectroscopy: a powerful tool for the non-contact discrimination of bone marrow mesenchymal stem cells and fibroblasts

Marieke Pudlas; Steffen Koch; Carsten Bolwien; Thomas Hirth; Heike Walles; Katja Schenke-Layland

doi:10.1117/12.873047

11 February 2011 Raman spectroscopy: a powerful tool for the non-contact discrimination of bone marrow mesenchymal stem cells and fibroblasts

Marieke Pudlas, Steffen Koch, Carsten Bolwien, Thomas Hirth, Heike Walles, Katja Schenke-Layland

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79032I (2011) https://doi.org/10.1117/12.873047
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Bone-marrow mesenchymal stem cells (BM-MSCs) are a promising cell source for regenerative medicine. However, it is challenging to determine whether isolated BM-MSC populations are free of fibroblasts. We employed traditional methods and Raman spectroscopy to distinguish BM-MSCs and human dermal fibroblasts (hDFs). Although in vitro differentiation assays revealed the multipotent character of BM-MSCs, long culture periods are a major disadvantage. Using Raman spectroscopy, we could quickly distinguish between BM-MSCs and hDFs. Therefore we conclude that this method is sufficient for the rapid detection of fibroblastic contaminations in BM-MSC cultures.

Citation Download Citation

Marieke Pudlas, Steffen Koch, Carsten Bolwien, Thomas Hirth, Heike Walles, and Katja Schenke-Layland "Raman spectroscopy: a powerful tool for the non-contact discrimination of bone marrow mesenchymal stem cells and fibroblasts", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032I (11 February 2011); https://doi.org/10.1117/12.873047

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