Delivery and characterization of sub-8fs laser pulses at the imaging plane of a two-photon microscope

Dmitry Pestov; Bingwei Xu; Haowen Li; Marcos Dantus

doi:10.1117/12.889223

28 February 2011 Delivery and characterization of sub-8fs laser pulses at the imaging plane of a two-photon microscope

Dmitry Pestov, Bingwei Xu, Haowen Li, Marcos Dantus

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 79033B (2011) https://doi.org/10.1117/12.889223
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We demonstrate a modular and versatile experimental setup that enables straightforward compression (and then shaping) of ultrashort laser pulses at the imaging plane of a two-photon microscope. A commercially available pulse shaper is used in conjunction with a commercially available broadband Ti:Sapphire oscillator to produce sub-8fs pulses at the focus of a high-numerical-aperture objective. Automated adaptive pulse compression, based on multiphoton intrapulse interference phase scan (MIIPS), is verified in situ by shaper assisted interferometric autocorrelation. Two-photon excited fluorescence image of a mouse kidney slide is obtained to confirm microscopic sectioning capabilities.

Citation Download Citation

Dmitry Pestov, Bingwei Xu, Haowen Li, and Marcos Dantus "Delivery and characterization of sub-8fs laser pulses at the imaging plane of a two-photon microscope", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79033B (28 February 2011); https://doi.org/10.1117/12.889223

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available