You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.
11 February 2011In-line digital holographic microscopy based on intensity
measurements at two planes
We propose a new approach of in-line digital holographic microscopy (DHM) with the capability of enhancing the
hologram acquisition rate together with improved reconstruction capability. The method is based on the recording of two
interferograms of the same object at slightly different planes. The technique utilizes the full spatial bandwidth of the
camera and do not require phase-shifting of the reference beam. Furthermore, we exploit the method of subtraction of
average intensity of the entire hologram to suppress the zero-order diffracted wave. The twin image is eliminated by
Fourier domain processing of the two recorded holograms. Experimental results of both amplitude and phase objects
demonstrate the feasibility of this method. Since the two interferograms can be recorded simultaneously by using two
CCD or CMOS sensors, this new in-line DHM technique has the potential applications in biomedical research for the
visualization of rapid dynamic processes at cellular level.
The alert did not successfully save. Please try again later.
Bhargab Das, Chandra S. Yelleswarapu, D. V. G. L. N. Rao, "In-line digital holographic microscopy based on intensity measurements at two planes," Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790403 (11 February 2011); https://doi.org/10.1117/12.875449