Adaptive optics for wide-field microscopy

C. Bourgenot; C. D. Saunter; J. M. Girkin; G. D. Love

doi:10.1117/12.873857

23 February 2011 Adaptive optics for wide-field microscopy

C. Bourgenot, C. D. Saunter, J. M. Girkin, G. D. Love

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 790414 (2011) https://doi.org/10.1117/12.873857
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

Citation Download Citation

C. Bourgenot, C. D. Saunter, J. M. Girkin, and G. D. Love "Adaptive optics for wide-field microscopy", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790414 (23 February 2011); https://doi.org/10.1117/12.873857

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