23 February 2011 Fourier analysis of mitochondrial distribution in oocytes
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This paper describes a novel approach to quantifying mitochondrial patterns which are typically described using the qualitative terms "diffuse" "aggregated" and are potentially key indicators for an oocyte's health and survival potential post-implantation. An oocyte was isolated in a confocal image and a coarse grid was superimposed upon it. The spatial spectrum was calculated and an aggregation factor was generated. A classifier for healthy cells was developed and verified. The aggregation factor showed a clear distinction between the healthy and unhealthy oocytes. The ultimate goal is to screen oocytes for viability preimplantation, thus improving the outcome of in vitro fertilization (IVF) treatments.
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Joseph L. Hollmann, Joseph L. Hollmann, Dana H. Brooks, Dana H. Brooks, Judith A. Newmark, Judith A. Newmark, Carol M. Warner, Carol M. Warner, Charles A. DiMarzio, Charles A. DiMarzio, } "Fourier analysis of mitochondrial distribution in oocytes", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790419 (23 February 2011); doi: 10.1117/12.876345; https://doi.org/10.1117/12.876345

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