23 February 2011 Plasma membrane microorganization of LR73 multidrug-resistant cells revealed by FCS
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Abstract
Tumoral cells could present a multidrug resistance (MDR) to chemotherapeutic treatments. This drug resistance would be associated to biomechanisms occurring at the plasma membrane level, involving modification of membrane fluidity, drug permeability, presence of microdomains (rafts, caveolae...), and membrane proteins overexpression such as Pglycoprotein. Fluorescence correlation spectroscopy (FCS) is the relevant method to investigate locally the fluidity of biological membranes through the lateral diffusion of a fluorescent membrane probe. Thus, we use FCS to monitor the plasma membrane local organization of LR73 carcinoma cells and three derived multidrug-resistant cancer cells lines. Measurements were conducted at the single cell level, which enabled us to get a detailed overview of the plasma membrane microviscosity distribution of each cell line studied. Moreover, we propose 2D diffusion simulation based on a Monte Carlo model to investigate the membrane organisation in terms of microdomains. This simulation allows us to relate the differences in the fluidity distributions with microorganization changes in plasma membrane of MDR cells.
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Pascale Winckler, Pascale Winckler, Rodolphe Jaffiol, Rodolphe Jaffiol, Aurélie Cailler, Aurélie Cailler, Hamid Morjani, Hamid Morjani, Pierre Jeannesson, Pierre Jeannesson, Régis Deturche, Régis Deturche, } "Plasma membrane microorganization of LR73 multidrug-resistant cells revealed by FCS", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050I (23 February 2011); doi: 10.1117/12.874496; https://doi.org/10.1117/12.874496
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