23 February 2011 Detection of pathogenic DNA at the single-molecule level
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Proceedings Volume 7905, Single Molecule Spectroscopy and Imaging IV; 79050M (2011); doi: 10.1117/12.873850
Event: SPIE BiOS, 2011, San Francisco, California, United States
Abstract
We demonstrate ultrasensitive detection of pathogenic DNA in a homogeneous assay at the single-molecule level applying two-color coincidence analysis. The target molecule we quantify is a 100 nucleotide long synthetic single-stranded oligonucleotide adapted from Streptococcus pneumoniae, a bacterium causing lower respiratory tract infections. Using spontaneous hybridization of two differently fluorescing Molecular Beacons we demonstrate a detection sensitivity of 100 fM (10-13M) in 30 seconds applying a simple microfluidic device with a 100 μm channel and confocal two-color fluorescence microscopy.
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Idir Yahiatène, Tobias Klamp, Mark Schüttpelz, Markus Sauer, "Detection of pathogenic DNA at the single-molecule level", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050M (23 February 2011); doi: 10.1117/12.873850; https://doi.org/10.1117/12.873850
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KEYWORDS
Luminescence

Molecules

Pathogens

Target detection

Microfluidics

Confocal microscopy

Quenching (fluorescence)

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