10 February 2011 Thin wetting film lensless imaging
Author Affiliations +
Abstract
Lensless imaging has recently attracted a lot of attention as a compact, easy-to-use method to image or detect biological objects like cells, but failed at detecting micron size objects like bacteria that often do not scatter enough light. In order to detect single bacterium, we have developed a method based on a thin wetting film that produces a micro-lens effect. Compared with previously reported results, a large improvement in signal to noise ratio is obtained due to the presence of a micro-lens on top of each bacterium. In these conditions, standard CMOS sensors are able to detect single bacterium, e.g. E.coli, Bacillus subtilis and Bacillus thuringiensis, with a large signal to noise ratio. This paper presents our sensor optimization to enhance the SNR; improve the detection of sub-micron objects; and increase the imaging FOV, from 4.3 mm2 to 12 mm2 to 24 mm2, which allows the detection of bacteria contained in 0.5μl to 4μl to 10μl, respectively.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
C. P. Allier, V. Poher, J. G. Coutard, G. Hiernard, J. M. Dinten, "Thin wetting film lensless imaging", Proc. SPIE 7906, Optical Diagnostics and Sensing XI: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue III, 790608 (10 February 2011); doi: 10.1117/12.874876; https://doi.org/10.1117/12.874876
PROCEEDINGS
8 PAGES


SHARE
RELATED CONTENT

Wide-field microscopy using microcamera arrays
Proceedings of SPIE (February 22 2013)
A high performance multi tap CMOS lock in pixel image...
Proceedings of SPIE (February 22 2017)
Lensfree in-line holographic detection of bacteria
Proceedings of SPIE (June 08 2011)
Characterization of biofilms with a fiber optic spectrometer
Proceedings of SPIE (December 15 2000)
A luminescent nisin biosensor
Proceedings of SPIE (February 23 2006)

Back to Top