Thin wetting film lensless imaging

C. P. Allier; V. Poher; J. G. Coutard; G. Hiernard; J. M. Dinten

doi:10.1117/12.874876

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10 February 2011 Thin wetting film lensless imaging

C. P. Allier, V. Poher, J. G. Coutard, G. Hiernard, J. M. Dinten

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Proceedings Volume 7906, Optical Diagnostics and Sensing XI: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue III; 790608 (2011) https://doi.org/10.1117/12.874876
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Lensless imaging has recently attracted a lot of attention as a compact, easy-to-use method to image or detect biological objects like cells, but failed at detecting micron size objects like bacteria that often do not scatter enough light. In order to detect single bacterium, we have developed a method based on a thin wetting film that produces a micro-lens effect. Compared with previously reported results, a large improvement in signal to noise ratio is obtained due to the presence of a micro-lens on top of each bacterium. In these conditions, standard CMOS sensors are able to detect single bacterium, e.g. E.coli, Bacillus subtilis and Bacillus thuringiensis, with a large signal to noise ratio. This paper presents our sensor optimization to enhance the SNR; improve the detection of sub-micron objects; and increase the imaging FOV, from 4.3 mm² to 12 mm² to 24 mm², which allows the detection of bacteria contained in 0.5μl to 4μl to 10μl, respectively.

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C. P. Allier, V. Poher, J. G. Coutard, G. Hiernard, and J. M. Dinten "Thin wetting film lensless imaging", Proc. SPIE 7906, Optical Diagnostics and Sensing XI: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue III, 790608 (10 February 2011); https://doi.org/10.1117/12.874876

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