Near-infrared scattering imaging of depolarization waves in a rat hypoxic brain model and its application to assessment of brain tissue reversibility

Satoko Kawauchi; Shunichi Sato; Yoichi Uozumi; Hiroshi Nawashiro; Miya Ishihara M.D.; Makoto Kikuchi M.D.

doi:10.1117/12.874615

11 February 2011 Near-infrared scattering imaging of depolarization waves in a rat hypoxic brain model and its application to assessment of brain tissue reversibility

Satoko Kawauchi, Shunichi Sato, Yoichi Uozumi, Hiroshi Nawashiro, Miya Ishihara M.D., Makoto Kikuchi M.D.

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Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 79070M (2011) https://doi.org/10.1117/12.874615
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Light scattering signal, which is sensitive to cellular/subcellular structural integrity, is a potential indicator of tissue viability in brain, because metabolic energy is used in part to maintain the structure of the cells. We performed near-infrared scattering imaging of rat brain during hypoxia followed by reoxygenation to examine spatiotemporal scattering change due to anoxic depolarization and its correlation with tissue reversibility. For imaging change in light scattering of rat brain, NIR light was transmitted from a halogen lamp with a bandpass filter (800 ± 70 nm) and it was incident onto the entire cortex through the intact skull at an oblique angle; diffusely reflected light from the brain was imaged with a charge-coupled device. About 2 min after starting hypoxia, scattering waves were generated focally in the bilateral outermost regions in the cortex and spread toward the midline at a rate of ~6 mm/min. When reoxygenation was started before the leading edges of scattering waves reached the midline of the brain, the scattering waves disappeared gradually and the tissue was saved. Reoxygenation when scattering wave reached the midline did not save the brain. These results suggest that the coverage of the scattering waves determine the brain tissue reversibility after hypoxia.

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Satoko Kawauchi, Shunichi Sato, Yoichi Uozumi, Hiroshi Nawashiro, Miya Ishihara M.D., and Makoto Kikuchi M.D. "Near-infrared scattering imaging of depolarization waves in a rat hypoxic brain model and its application to assessment of brain tissue reversibility", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070M (11 February 2011); https://doi.org/10.1117/12.874615

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KEYWORDS

Brain

Scattering

Light scattering

Neuroimaging

Tissues

Hypoxia

Near infrared

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