11 February 2011 Variations in the optical scattering properties of skin in murine animal models
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In the work presented here, the optical scattering properties of mouse skin are investigated in depth with the use of Elastic Scattering Spectroscopy (ESS). In particular, sources of variation that lead to experimental error are identified and examined. The thickness of the dermal layer of the skin is determined to be the primary source of variation due to its high collagen content. Specifically, gender differences in skin thickness are found to cause increases in the reflectance and scattering coefficient value by a factor of two in males as opposed to females. Changes in the hair growth cycle are found to influence scattering strength not only due to changes in skin thickness, but also from melanin collection in hair follicles. Because direct and/or indirect measurement of mouse skin is common in the development of novel biomedical optics techniques (optical biopsy, molecular imaging, in vivo monitoring of glucose/blood oxygenation, etc.), the purpose of this work is to identify sources of experimental variation that may arise in these studies such that care can be taken to avoid or compensate for their affects.
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Katherine Calabro, Katherine Calabro, Allison Curtis, Allison Curtis, Jean-Rene Galarneau, Jean-Rene Galarneau, Thomas Krucker, Thomas Krucker, Irving J. Bigio, Irving J. Bigio, } "Variations in the optical scattering properties of skin in murine animal models", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070S (11 February 2011); doi: 10.1117/12.880166; https://doi.org/10.1117/12.880166

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