Intrinsic optical signal imaging of glucose-stimulated physiological responses in the insulin secreting INS-1 &#946;-cell line

Yi-Chao Li; Wan-Xing Cui; Xu-Jing Wang; Franklin Amthor; Xin-Cheng Yao

doi:10.1117/12.874989

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11 February 2011 Intrinsic optical signal imaging of glucose-stimulated physiological responses in the insulin secreting INS-1 β-cell line

Yi-Chao Li, Wan-Xing Cui, Xu-Jing Wang, Franklin Amthor, Xin-Cheng Yao

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Proceedings Volume 7907, Biomedical Applications of Light Scattering V; 79070Y (2011) https://doi.org/10.1117/12.874989
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Intrinsic optical signal (IOS) imaging has been established for noninvasive monitoring of stimulus-evoked physiological responses in the retina and other neural tissues. Recently, we extended the IOS imaging technology for functional evaluation of insulin secreting INS-1 cells. INS-1 cells provide a popular model for investigating β-cell dysfunction and diabetes. Our experiments indicate that IOS imaging allows simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial (sub-cellular) and temporal (sub-second) resolution. Rapid image sequences reveal transient optical responses that have time courses comparable to glucose-evoked β-cell electrical activities.

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Yi-Chao Li, Wan-Xing Cui, Xu-Jing Wang, Franklin Amthor, and Xin-Cheng Yao "Intrinsic optical signal imaging of glucose-stimulated physiological responses in the insulin secreting INS-1 β-cell line", Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070Y (11 February 2011); https://doi.org/10.1117/12.874989

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