Imaging heterostructured quantum dots in cultured cells with epifluorescence and transmission electron microscopy

Erin M. Rivera; Casilda Trujillo Provencio; Andrea Steinbrueck; Pawan Rastogi; Allison Dennis; Jennifer Hollingsworth; Elba E. Serrano

doi:10.1117/12.875737

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11 February 2011 Imaging heterostructured quantum dots in cultured cells with epifluorescence and transmission electron microscopy

Erin M. Rivera, Casilda Trujillo Provencio, Andrea Steinbrueck, Pawan Rastogi, Allison Dennis, Jennifer Hollingsworth, Elba E. Serrano

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Proceedings Volume 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI; 79090N (2011) https://doi.org/10.1117/12.875737
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Quantum dots (QDs) are semiconductor nanocrystals with extensive imaging and diagnostic capabilities, including the potential for single molecule tracking. Commercially available QDs offer distinct advantages over organic fluorophores, such as increased photostability and tunable emission spectra, but their cadmium selenide (CdSe) core raises toxicity concerns. For this reason, replacements for CdSe-based QDs have been sought that can offer equivalent optical properties. The spectral range, brightness and stability of InP QDs may comprise such a solution. To this end, LANL/CINT personnel fabricated moderately thick-shell novel InP QDs that retain brightness and emission over time in an aqueous environment. We are interested in evaluating how the composition and surface properties of these novel QDs affect their entry and sequestration within the cell. Here we use epifluorescence and transmission electron microscopy (TEM) to evaluate the structural properties of cultured Xenopus kidney cells (A6; ATCC) that were exposed either to commercially available CdSe QDs (Qtracker® 565, Invitrogen) or to heterostructured InP QDs (LANL). Epifluorescence imaging permitted assessment of the general morphology of cells labeled with fluorescent molecular probes (Alexa Fluor® ® phalloidin; Hoechst 33342), and the prevalence of QD association with cells. In contrast, TEM offered unique advantages for viewing electron dense QDs at higher resolution with regard to subcellular sequestration and compartmentalization. Preliminary results show that in the absence of targeting moieties, InP QDs (200 nM) can passively enter cells and sequester nonspecifically in cytosolic regions whereas commercially available targeted QDs principally associate with membranous structures within the cell. Supported by: NIH 5R01GM084702.

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Erin M. Rivera, Casilda Trujillo Provencio, Andrea Steinbrueck, Pawan Rastogi, Allison Dennis, Jennifer Hollingsworth, and Elba E. Serrano "Imaging heterostructured quantum dots in cultured cells with epifluorescence and transmission electron microscopy", Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 79090N (11 February 2011); https://doi.org/10.1117/12.875737

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