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11 February 2011 Quantum dots as a FRET donor and nanoscaffold for multivalent DNA photonic wires
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We demonstrate Förster resonance energy transfer (FRET) through DNA photonic wires self-assembled around a central CdSe/ZnS semiconductor quantum dot (QD). The central QD acts as a nanoscaffold and FRET donor to a series of acceptor dyes along a DNA strand. By utilizing a DNA intercalating dye, altering the location of the dyes and using a series of increasingly red-shifted dyes along the DNA, we are able to track the efficiency of energy transfer through the DNA photonic structure via steady-state spectroscopy. Data suggests that limiting factors for efficient energy transfer are the sub-obtimal photophysical properties of acceptor dyes, including low quantum yields. These issues may be addressed with improved configurations of QDs, DNA and dyes. The development of biophotonic wire assemblies utilizing the superior photophysical properties of QDs will have widespread application in nanotechnology.
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Kelly Boeneman, Duane E. Prasuhn, Juan B. Blanco-Canosa, Philip E. Dawson, Joseph S. Melinger, Mario Ancona, Michael H. Stewart, Kimihiro Susumu, Alan Huston, and Igor L. Medintz "Quantum dots as a FRET donor and nanoscaffold for multivalent DNA photonic wires", Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 79090R (11 February 2011);

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