Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression

Jianpeng Xue; Lingling Shan; Zhenzhen Tu; Hongyan Zhu; Haiyan Chen; Dawei Deng; Zhiyu Qian; Yueqing Gu

doi:10.1117/12.874014

11 February 2011 Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression

Jianpeng Xue, Lingling Shan, Zhenzhen Tu, Hongyan Zhu, Haiyan Chen, Dawei Deng, Zhiyu Qian, Yueqing Gu

Proceedings Volume 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III; 79100I (2011) https://doi.org/10.1117/12.874014
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Signal transducer and activator of transcription (STAT) 5b is an important protein in JAK-STAT signal pathway and is responsible for the metastasis and proliferation of tumor cells. The determination of the STAT5b expression provides a way to study the mechanism of tumor progress. In this study, gold nanoparticles with different diameters were conjugated to the fluorescein modified STAT5b specific DNA sequence to form the beacon. The procedures for the beacon with better fluorescence properties were optimized. The fluorescence quenching and the recovery properties after hybridizing with mRNA of STAT5b were intensively investigated. Results indicate the gold nanoparticle based beacon is an effective probe for the determination of STAT5b protein expression in JAK-STAT signal pathway and has great potential in the study of drug screening and discovery.

Citation Download Citation

Jianpeng Xue, Lingling Shan, Zhenzhen Tu, Hongyan Zhu, Haiyan Chen, Dawei Deng, Zhiyu Qian, and Yueqing Gu "Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100I (11 February 2011); https://doi.org/10.1117/12.874014

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