11 February 2011 Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression
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Abstract
Signal transducer and activator of transcription (STAT) 5b is an important protein in JAK-STAT signal pathway and is responsible for the metastasis and proliferation of tumor cells. The determination of the STAT5b expression provides a way to study the mechanism of tumor progress. In this study, gold nanoparticles with different diameters were conjugated to the fluorescein modified STAT5b specific DNA sequence to form the beacon. The procedures for the beacon with better fluorescence properties were optimized. The fluorescence quenching and the recovery properties after hybridizing with mRNA of STAT5b were intensively investigated. Results indicate the gold nanoparticle based beacon is an effective probe for the determination of STAT5b protein expression in JAK-STAT signal pathway and has great potential in the study of drug screening and discovery.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jianpeng Xue, Jianpeng Xue, Lingling Shan, Lingling Shan, Zhenzhen Tu, Zhenzhen Tu, Hongyan Zhu, Hongyan Zhu, Haiyan Chen, Haiyan Chen, Dawei Deng, Dawei Deng, Zhiyu Qian, Zhiyu Qian, Yueqing Gu, Yueqing Gu, } "Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100I (11 February 2011); doi: 10.1117/12.874014; https://doi.org/10.1117/12.874014
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