Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima

Naveen K. Balla; Colin J. R. Sheppard; Peter T. C. So

doi:10.1117/12.876014

11 February 2011 Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima

Naveen K. Balla, Colin J. R. Sheppard, Peter T. C. So

Author Affiliations +

Proceedings Volume 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III; 79100N (2011) https://doi.org/10.1117/12.876014
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Gold nanoparticles are quite popular as contrast agents for optical microscopy. Their strong linear and nonlinear interaction with light, coupled with their biocompatibility and resistance to photobleaching make them suitable contrasts agents for bioimaging applications. Gold nanorods have been used for in vivo two photon microscopy in small animals [PNAS 102, 15752 (2005)]. Conventional two photon microscopy with gold nanorods involves exciting these particles with femtosecond laser at wavelengths close to their longitudinal plasmon resonance (LPR). Most of the reported works used Ti:Sapphire laser with excitation wavelengths ranging from 780 nm to 850 nm. The rational was to maximize absorption of excitation wavelengths, a fraction of which gives rise to two photon luminescence. This however causes intense heating of the nanorods and unless the excitation powers are kept low, gold nanorods tend to melt [Phys Rev Lett 95, 267405 (2005)]. Another less explored way of getting multiphoton emission from gold nanorods is to excite them at long wavelengths far away from their LPR wavelength [Jour Amer Chem Soc 131, 14186 (2009)]. We are interested in femtosecond lasers operating around 1200 nm wavelengths because of their lower scattering and absorption by tissue and water. Here we compare multiphoton photon luminescence properties of gold nanorods when excited at wavelengths around 800 nm and 1200 nm. Excitation with wavelengths around 1200 nm has certain advantages like lower heating of the particles and hence prolonged durations of imaging. Other advantage is the ability to collect emission in the near infrared regions (NIR) up to 800 nm which is not possible when using excitation wavelengths around 800 nm. These features are good for deep tissue imaging. One disadvantage of this approach is lower luminescence intensity.

Citation Download Citation

Naveen K. Balla, Colin J. R. Sheppard, and Peter T. C. So "Multiphoton luminescence of gold nanorods upon excitation with wavelengths away from their absorption maxima", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100N (11 February 2011); https://doi.org/10.1117/12.876014

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available