11 February 2011 Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine
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Abstract
In nanomedicine, biodistribution studies are critical to evaluate the safety and efficacy of nanoparticles. Currently, extensive biodistribution studies are hampered by the limitations of bulk tissue and single-cell imaging techniques. To ameliorate these limitations, we have developed a novel method for single nanoparticle detection that incorporates a conjugated oligonucleotide as a "nanobarcode" for detection via in situ PCR. This strategy magnifies the detection signal from single nanoparticles, facilitating rapid evaluation of nanoparticle uptake by cell type over larger areas. The nanobarcoding method can enable precise analysis of nanoparticle biodistributions and expedite translation of these nanoparticles to the clinic.
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Trisha Eustaquio, Trisha Eustaquio, Christy L. Cooper, Christy L. Cooper, James F. Leary, James F. Leary, } "Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100O (11 February 2011); doi: 10.1117/12.874290; https://doi.org/10.1117/12.874290
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