11 February 2011 Dye-biomolecule conjugates and NIR-fluorescent particles for targeting of disease-related biomarkers
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Abstract
Indispensable for fluorescence imaging are highly specific and sensitive molecular probes that absorb and emit in the near infrared (NIR) spectral region and respond to or target molecular species or processes. Here, we present approaches to targeted fluorescent probes for in vivo imaging in the intensity and lifetime domain exploiting NIR dyes. Screening schemes for the fast identification of suitable fluorophores are derived and design criteria for highly emissive optical probes. In addition, as a signal amplification strategy that enables also the use of hydrophobic NIR fluorophores as fluorescent reporters, first steps towards versatile strategies for the preparation of NIR-fluorescent polymeric particles are presented that can be utilized also for the design of targeted and analyte-responsive probes.
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J. Pauli, R. Brehm, M. Grabolle, T. Behnke, J. Mathejczyk, F. Hamann, F. Alves, I. Hilger, U. Resch-Genger, "Dye-biomolecule conjugates and NIR-fluorescent particles for targeting of disease-related biomarkers", Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 791014 (11 February 2011); doi: 10.1117/12.876828; https://doi.org/10.1117/12.876828
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