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11 February 2011 Enhanced SPR sensing based on micro-patterned thin films
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Proceedings Volume 7911, Plasmonics in Biology and Medicine VIII; 79110B (2011)
Event: SPIE BiOS, 2011, San Francisco, California, United States
Micro-patterned thin films interrogated in the Kretschmann configuration of SPR could extend the detection range to lower concentrations and small biomolecules due to a greater sensitivity. This was achieved with the same instrumentation and analysis methodologies developed for SPR with continuous films. The plasmonic properties of micro-patterned thin films composed of various layers of Ag and Au were investigated to find an optimal structure for biosensing application. The analytical parameters and biosensing performances were also evaluated for analysis of biological samples. Au microhole arrays of 3.2 μm periodicity and 1.6 μm hole diameter were prepared using a modified nanosphere lithography (NSL) technique. These microstructures showed optimal plasmonic properties for biosensing applications as they exhibit a 50% increase in sensitivity to refractive index changes compared to continuous thin films of the same thickness. Moreover, microhole arrays presented a faster response time to refractive index changes while analytical parameters such as the resolution and the noise in biosensing measurement were comparable to continuous films. When combined to the appropriate surface chemistry, a greater SPR response was measured for proteins using microhole arrays. Although microhole arrays required an additional preparation step, a cleaning step using oxygen plasma allowed multiple measurements with the same metallic surface with great repeatability. Hence, microhole arrays proved to be a simple approach to improve current SPR biosensing technique. Further investigations to understand the plasmonic properties of microhole were performed using an angle scanning SPR instrument.
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Ludovic S. Live, Julien Breault-Turcot, Kim-Ly Nguyen, and Jean-Francois Masson "Enhanced SPR sensing based on micro-patterned thin films", Proc. SPIE 7911, Plasmonics in Biology and Medicine VIII, 79110B (11 February 2011);

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