Multispectral imaging is becoming a new powerful tool in a wide range of biological studies by adding spectral, spatial
and temporal dimensions to tissue abnormity and the underlying biological processes. A standard spectral imaging setup
includes two major components, a band pass selection device (such as liquid crystal tunable filter and diffraction grating)
and a scientific-grade monochrome camera. Contemporary multispectral imaging technologies typically use traditional
optical filters e.g., filter wheels, a generalized Lyot filter, an electrically tunable filter, multiple-band pass filters or the
methods of dispersing light, e.g., optic-acoustic crystals. The instrumented systems are bulky, expensive, require
multiple exposures or extensive post-processing to align up multiple images of pure spectral components.
Recently a break-through technology has emerged to instrument multispectral imaging technology into handheld real-time
devices using miniaturized filter mosaic containing micro-arrayed multiple channel band-pass optical filters. The
filter mosaic can be directly placed near the focal plane immediately in front of the imaging sensor of an off-shelf
CCD/CMOS camera, with potentially one such micro-filter covers one pixel of the imaging sensor. This paper reveals
the technical details of how such a micro-arrayed multi channel optical filter is fabricated using traditional multi-film
vacuum deposition and the modern micro-lithography technologies. The selection of different coating materials, their
structures and effects to the adhesive forces between film and substrate, the spatial resolution, width of passing band, and
the transmittance of the resulting miniaturized optical filter is discussed.