11 February 2011 Integrated lab-on-a-chip: a combined sample preparation and PCR system as an ultrafast analytical tool for pathogen detection
Author Affiliations +
Proceedings Volume 7929, Microfluidics, BioMEMS, and Medical Microsystems IX; 792903 (2011); doi: 10.1117/12.874551
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States
Abstract
Lab-on-a-chip systems are seen as a very promising approach for a decentralized continuous pathogen monitoring technology. In this paper, we present the development of a fully integrated device for the multiplexed nucleic-acid based identification of pathogens. Due to the complexity of such a fully integrated device, in a first development step, functional modules for the various process steps like lysis, DNA extraction and purification, continuous-flow PCR and detection have been developed and evaluated, allowing a functional verification prior to integration. All the modules as well as the final integrated device have been manufactured using scaleable industrial manufacturing methods, namely injection molding in order to facilitate commercialization.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Holger Becker, Nadine Hlawatsch, Richard Klemm, Claudia Gärtner, "Integrated lab-on-a-chip: a combined sample preparation and PCR system as an ultrafast analytical tool for pathogen detection", Proc. SPIE 7929, Microfluidics, BioMEMS, and Medical Microsystems IX, 792903 (11 February 2011); doi: 10.1117/12.874551; http://dx.doi.org/10.1117/12.874551
PROCEEDINGS
5 PAGES


SHARE
KEYWORDS
Pathogens

Microfluidics

Manufacturing

Lab on a chip

Control systems

Statistical analysis

Bacteria

RELATED CONTENT

The photon: EM fields, electrical potentials, and AC charge
Proceedings of SPIE (September 10 2015)
Mask cleaning strategies: haze elimination
Proceedings of SPIE (November 08 2005)
Statistical assessment of model fit for SAR sea clutter
Proceedings of SPIE (October 30 2009)
3D luminescent photonic crystal structures
Proceedings of SPIE (May 25 2005)

Back to Top