14 February 2011 Pupil-segmentation-based adaptive optics for microscopy
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Proceedings Volume 7931, MEMS Adaptive Optics V; 79310I (2011) https://doi.org/10.1117/12.876398
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States
Abstract
Inhomogeneous optical properties of biological samples make it difficult to obtain diffraction-limited resolution in depth. Correcting the sample-induced optical aberrations needs adaptive optics (AO). However, the direct wavefront-sensing approach commonly used in astronomy is not suitable for most biological samples due to their strong scattering of light. We developed an image-based AO approach that is insensitive to sample scattering. By comparing images of the sample taken with different segments of the pupil illuminated, local tilt in the wavefront is measured from image shift. The aberrated wavefront is then obtained either by measuring the local phase directly using interference or with phase reconstruction algorithms similar to those used in astronomical AO. We implemented this pupil-segmentation-based approach in a two-photon fluorescence microscope and demonstrated that diffraction-limited resolution can be recovered from nonbiological and biological samples.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Na Ji, Na Ji, Daniel E. Milkie, Daniel E. Milkie, Eric Betzig, Eric Betzig, } "Pupil-segmentation-based adaptive optics for microscopy", Proc. SPIE 7931, MEMS Adaptive Optics V, 79310I (14 February 2011); doi: 10.1117/12.876398; https://doi.org/10.1117/12.876398
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