14 February 2011 Adaptive optics wide-field microscope corrections using a MEMS DM and Shack-Hartmann wavefront sensor
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Proceedings Volume 7931, MEMS Adaptive Optics V; 79310J (2011) https://doi.org/10.1117/12.876439
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States
Abstract
We demonstrated the used of an adaptive optic system in biological imaging to improve the imaging characteristics of a wide field microscope. A crimson red fluorescent bead emitting light at 650 nm was used together with a Shack-Hartmann wavefront sensor and deformable mirror to compensate for the aberrations introduce by a Drosophila embryo. The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved (510 nm). The use of the crimson beads allow for less photobleaching to be done to the science object of the embryo, in this case our GFP model (green fluorescent beads), and allows for the science object and wavefront reference to be spectrally separated. The spectral separation allows for single points sources to be used for wavefront measurements, which is a necessary condition for the Shack-Hartmann Wavefront sensor operation.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Oscar Azucena, Oscar Azucena, Xiaodong Tao, Xiaodong Tao, Justin Crest, Justin Crest, Shaila Kotadia, Shaila Kotadia, William Sullivan, William Sullivan, Donald Gavel, Donald Gavel, Marc Reinig, Marc Reinig, Scot Olivier, Scot Olivier, Joel Kubby, Joel Kubby, } "Adaptive optics wide-field microscope corrections using a MEMS DM and Shack-Hartmann wavefront sensor", Proc. SPIE 7931, MEMS Adaptive Optics V, 79310J (14 February 2011); doi: 10.1117/12.876439; https://doi.org/10.1117/12.876439
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