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14 February 2011 Adaptive optics in wide-field microscopy
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Proceedings Volume 7931, MEMS Adaptive Optics V; 79310K (2011)
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States
Three-dimensional live imaging in cell biology is hindered by optical aberrations which degrade the resolution and signal-to-noise ratio as the focal plane is moved deeper into the sample. The solution to this problem is to use adaptive optics to correct the aberrations. In this paper, we discuss our work on applying adaptive optics to wide-field fluorescence microscopy. We demonstrate correction of depth-aberrations and focusing using a deformable mirror in open-loop operation. We then discuss the use of phase retrieval and phase diversity in adaptive optics.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Peter Kner, Zvi Kam, David Agard, and John Sedat "Adaptive optics in wide-field microscopy", Proc. SPIE 7931, MEMS Adaptive Optics V, 79310K (14 February 2011);

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