Generation-3 programmable array microscope (PAM) with digital micro-mirror device (DMD)

Pieter A. A. De Beule; Anthony H. B. de Vries; Donna J. Arndt-Jovin; Thomas M. Jovin

doi:10.1117/12.879611

14 February 2011 Generation-3 programmable array microscope (PAM) with digital micro-mirror device (DMD)

Pieter A. A. De Beule, Anthony H. B. de Vries, Donna J. Arndt-Jovin, Thomas M. Jovin

Proceedings Volume 7932, Emerging Digital Micromirror Device Based Systems and Applications III; 79320G (2011) https://doi.org/10.1117/12.879611
Event: SPIE MOEMS-MEMS, 2011, San Francisco, California, United States

Abstract

We report progress on the construction of an optical sectioning programmable array microscope (PAM) implemented with a digital micro-mirror device (DMD) spatial light modulator (SLM) utilized for both fluorescence illumination and detection. The introduction of binary intensity modulation at the focal plane of a microscope objective in a computer controlled pixilated mode allows the recovery of an optically sectioned image. Illumination patterns can be changed very quickly, in contrast to static Nipkow disk or aperture correlation implementations, thereby creating an optical system that can be optimized to the optical specimen in a convenient manner, e.g. for patterned photobleaching, photobleaching reduction, or spatial superresolution. We present a third generation (Gen-3) dual path PAM module incorporating the 25 kHz binary frame rate TI 1080p DMD and a newly developed optical system that offers diffraction limited imaging with compensation of tilt angle distortion.

Citation Download Citation

Pieter A. A. De Beule, Anthony H. B. de Vries, Donna J. Arndt-Jovin, and Thomas M. Jovin "Generation-3 programmable array microscope (PAM) with digital micro-mirror device (DMD)", Proc. SPIE 7932, Emerging Digital Micromirror Device Based Systems and Applications III, 79320G (14 February 2011); https://doi.org/10.1117/12.879611

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