1 March 2011 Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors
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Abstract
We developed ratiometric optical oxygen sensors to probe the oxygen consumption during epileptic events in rat brain slices. The oxygen sensors consist of the sensing part of phosphorescence dyes (Platinum (II) octaethylporphine ketone) and reference part of nanocystal quantum dots (NQDs) embedded in polymer blends, with pre-designed excitation through fluorescence resonance energy transfer (FRET) from NQDs to the oxygen sensitive dyes (OSDs). The ratiometric FRET sensors with fast temporal response and excellent bio-compatibility are suitable for real time quantitative dissolved oxygen (D.O.) probes in biological microenvironment. Coating the sensors onto the micro-pipettes, we performed simultaneous oxygen probes at pyramidal and oriens layers in rat hippocampal CA1. Different spatiotemporal patterns with maximum D.O. decreases of 9.9±1.1 mg/L and 4.9±0.7 mg/L during seizure events were observed in pyramidal and oriens layers, respectively.
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Chunfeng Zhang, Chunfeng Zhang, Justin Ingram, Justin Ingram, Steven Schiff, Steven Schiff, Jian Xu, Jian Xu, Min Xiao, Min Xiao, } "Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors", Proc. SPIE 7947, Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII, 79470B (1 March 2011); doi: 10.1117/12.876341; https://doi.org/10.1117/12.876341
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