Hybrid optical transport trap: loading and unloading of microscale objects using a microfabricated optical fiber into optical tweezers

Nelson Cardenas; Yogeshwar N. Mishra; Samarendra K. Mohanty

doi:10.1117/12.875954

16 February 2011 Hybrid optical transport trap: loading and unloading of microscale objects using a microfabricated optical fiber into optical tweezers

Nelson Cardenas, Yogeshwar N. Mishra, Samarendra K. Mohanty

Author Affiliations +

Proceedings Volume 7950, Complex Light and Optical Forces V; 795008 (2011) https://doi.org/10.1117/12.875954
Event: SPIE OPTO, 2011, San Francisco, California, United States

Abstract

High throughput analysis of trapped samples requires effective loading and unloading into the trap in a microfluidic environment. We demonstrate development of a hybrid optical transport trap (HOTT) which combines a tapered fiberoptic 2D trap for transport of microscopic objects into and out of the optical tweezers trap in an orthogonal geometry. For small cone angle of the tip, the microscopic objects (polystyrene and red blood cells) were found to be trapped in two-dimensions and pushed along the axial direction by domination of scattering force. This was found to be in consistence with the estimated axial forces caused by the beam profiles emerging from the small-cone tapered fiber tip. While for loading of the microscopic objects into the optical tweezers trap, the fiber tip was placed ~ 30μm away from the tweezers trap, unloading was carried out in presence of the tip close (<15 μm) to the tweezers trap. Further, for a fixed fiber trap and tweezers separation (~ 30 μm), both loading and unloading could be achieved by reducing the tweezers trap power so that the scattering force exerted by the fiber trap exceeded the transverse gradient force of tweezers trap. Since the tapered tip can be easily integrated onto a microfluidic channel, the proposed configuration can find potential applications in lab-on-a-chip devices. We demonstrate analysis of transported microscopic objects using digital holographic microscopy integrated with the HOTT.

Citation Download Citation

Nelson Cardenas, Yogeshwar N. Mishra, and Samarendra K. Mohanty "Hybrid optical transport trap: loading and unloading of microscale objects using a microfabricated optical fiber into optical tweezers", Proc. SPIE 7950, Complex Light and Optical Forces V, 795008 (16 February 2011); https://doi.org/10.1117/12.875954

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Axicons

Optical tweezers

Particles

Digital holography

Holography

Scattering

Optical fibers

Show All Keywords

Keywords/Phrases

Search In:

Publication Years