Assessment of a CAD scheme in selecting the optimal focused microscopic scanning images of the metaphase chromosomes

Xingwei Wang; Jun Tan; Yuchen Qiu; Yuhua Li; Hong Liu; Shibo Li; Bin Zheng

doi:10.1117/12.876864

3 March 2011 Assessment of a CAD scheme in selecting the optimal focused microscopic scanning images of the metaphase chromosomes

Xingwei Wang, Jun Tan, Yuchen Qiu, Yuhua Li, Hong Liu, Shibo Li, Bin Zheng

Author Affiliations +

Proceedings Volume 7966, Medical Imaging 2011: Image Perception, Observer Performance, and Technology Assessment; 796618 (2011) https://doi.org/10.1117/12.876864
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

Visually searching for analyzable metaphase chromosome cells under microscopes is a routine and timeconsuming task in genetic laboratories to diagnose cancer and genetic disorders. To improve detection efficiency, consistency, and accuracy, we developed an automated microscopic image scanning system using a 100X oil immersion objective lens to acquire images that has sufficient spatial resolution allowing clinicians to do diagnosis. Due to the highresolution, the field of image depth is very limited and multiple scans up to seven layers are required. Thus, a metaphase cell can spread over multiple images at different focal levels. Among them only one or two are adequate for the diagnosis and the others are typically fuzzy images. In this study, we developed and tested a computer-aided detection (CAD) scheme to automatically select one image with the sharpest image quality and discard all of the other fuzzy images based on the computed sharpness index. From three scanned bone marrow specimen slides, the on-line and offline metaphase finding modules automatically selected 100 chromosome cells with 534 images. These images were selected to build a testing dataset. For each cell, the CAD scheme selects one image with the maximum sharpness index. Three observers also independently visually selected one best image for diagnosis from each cell. The agreement rate between CAD and visually selected images ranges from 89% to 96%, which is also very comparable to the agreement rate between the two observers. This experiment demonstrated the feasibility of applying a CAD scheme to select the images with sharpest high-resolution metaphase chromosome cell and potentially improve diagnostic efficiency and accuracy in the future clinical practice.

Citation Download Citation

Xingwei Wang, Jun Tan, Yuchen Qiu, Yuhua Li, Hong Liu, Shibo Li, and Bin Zheng "Assessment of a CAD scheme in selecting the optimal focused microscopic scanning images of the metaphase chromosomes", Proc. SPIE 7966, Medical Imaging 2011: Image Perception, Observer Performance, and Technology Assessment, 796618 (3 March 2011); https://doi.org/10.1117/12.876864

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Computer aided diagnosis and therapy

Image segmentation

Genetics

Image processing

Visualization

Cancer

Bone

Show All Keywords

Keywords/Phrases

Search In:

Publication Years