Optical disc drives are inexpensive, readily available, and use highly sophisticated optoelectronic components which can
be adapted for sensing. One limitation of using compact discs (CDs) and optical disk drives for sensing of analytes
placed on a CD is the fluctuations in the voltage signal from the disk drive generated while reading the data on the CD.
In this study, we develop a simple, low-cost strategy for sensing and identification using CDs and optical disk drives that
spectrally separates contributions to the voltage signal caused by an analyte intentionally placed onto the CD and that
caused by the underlying data on the CD. Analytes are printed onto a CD surface with fixed spatial periodicity. As the
laser beam in an optical disk drive scans over the section of the CD containing the analyte pattern, the intensity of the
laser beam incident onto the photodiode integrated into the disk drive is modulated at a frequency dependent on the
spatial periodicity of the analyte pattern and the speed of the optical disk drive motor. Fourier transformation of the
voltage signal from the optical disk drive yields peaks in the frequency spectrum with amplitudes and locations that
enable analyte sensing and identification, respectively. We study the influence of analyte area coverage, pattern
periodicity, and CD rotational frequency on the peaks in the frequency spectrum associated with the patterned analyte.
We apply this technique to discriminate differently-coloured analytes, perform trigger-free detection of multiple analytes
distributed on a single CD, and detect at least two different, overlapped analyte patterns on a single CD. The extension
of this technique for sensing and identification of colorimetric chemical reagents is discussed. Future work will focus on
adapting this technique to perform measurements at multiple wavelengths and streamlining the data collection and