High topographical accuracy by optical noise reduction in digital holographic microscopy

Miguel León-Rodríguez; Ramón Rodríguez-Vera; Juan A. Rayas; Sergio Calixto

doi:10.1117/12.902595

25 October 2011 High topographical accuracy by optical noise reduction in digital holographic microscopy

Miguel León-Rodríguez, Ramón Rodríguez-Vera, Juan A. Rayas, Sergio Calixto

Proceedings Volume 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World; 801116 (2011) https://doi.org/10.1117/12.902595
Event: International Commission for Optics (ICO 22), 2011, Puebla, Mexico

Abstract

In this work we present a different method to reduce shot noise in phase imaging from digital holograms. An averaging process of phase images reconstructed with different reconstruction algorithms of the complex amplitude of a phase object in digital holographic microscopy. We obtain an improved phase image reaching a 29% of shot noise reduction. We use a single object complex amplitude that is needed to perform our proposal. Also show the corresponding simulations and experimental results. As phase sample test we used a micro-thin film step surface made at home of 100 nm high of TiO₂ on a glass substrate of 4.7 mm thickness, our system was calibrated and traceable to an Atomic Force Microscope results.

Citation Download Citation

Miguel León-Rodríguez, Ramón Rodríguez-Vera, Juan A. Rayas, and Sergio Calixto "High topographical accuracy by optical noise reduction in digital holographic microscopy", Proc. SPIE 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World, 801116 (25 October 2011); https://doi.org/10.1117/12.902595

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KEYWORDS

Digital holography

Holograms

3D image reconstruction

Image processing

Denoising

Photons

Optical simulations

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