THz spectroscopy based high sensitivity measurement of protein using a metal mesh device

T. Suzuki; Y. Ogawa; T. Kondo; S. Kamba; N. Kondo

doi:10.1117/12.886691

2 June 2011 THz spectroscopy based high sensitivity measurement of protein using a metal mesh device

T. Suzuki, Y. Ogawa, T. Kondo, S. Kamba, N. Kondo

Proceedings Volume 8027, Sensing for Agriculture and Food Quality and Safety III; 802706 (2011) https://doi.org/10.1117/12.886691
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

A label-free bioaffinity sensor working in terahertz (THz) region with a nitrocellulose membrane filter was demonstrated, which is based on the resonant transmission phenomenon and the dip in the spectra of the metal mesh device. By using this sensor, we succeeded in the highly sensitive detection of small amounts of protein avidin-biotin complex. A distinct change of transmittance caused by shift of the transmission dip was observed for 8 ng/mm² (74 fmol) of horseradish peroxidase (HRP) labeled avidin. The sensing method has broad utility for many reactions on the membrane filter as a simple and rapid sensor.

Citation Download Citation

T. Suzuki, Y. Ogawa, T. Kondo, S. Kamba, and N. Kondo "THz spectroscopy based high sensitivity measurement of protein using a metal mesh device", Proc. SPIE 8027, Sensing for Agriculture and Food Quality and Safety III, 802706 (2 June 2011); https://doi.org/10.1117/12.886691

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