The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor

Yating Chai; Suiqiong Li; Shin Horikawa; Wen Shen; Mi-Kyung Park; Vitaly J. Vodyanoy; Bryan A. Chin

doi:10.1117/12.883705

2 June 2011 The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor

Yating Chai, Suiqiong Li, Shin Horikawa, Wen Shen, Mi-Kyung Park, Vitaly J. Vodyanoy, Bryan A. Chin

Proceedings Volume 8027, Sensing for Agriculture and Food Quality and Safety III; 802708 (2011) https://doi.org/10.1117/12.883705
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

This paper presents the direct detection of Salmonella typhimurium on shell eggs using a phage-based magnetoelastic (ME) biosensor. The ME biosensor consists of a ME resonator as the sensor platform and E2 phage as the biorecognition element that is genetically engineered to specifically bind with Salmonella typhimurium. The ME biosensor, which is a wireless sensor, vibrates with a characteristic resonant frequency under an externally applied magnetic field. Multiple sensors can easily be remotely monitored. Multiple measurement and control sensors were placed on the shell eggs contaminated by Salmonella typhimurium solutions with different known concentrations. The resonant frequency of sensors before and after the exposure to the spiked shell eggs was measured. The frequency shift of the measurement sensors was significantly different than the control sensors indicating Salmonella contamination. Scanning electron microscopy was used to confirm binding of Salmonella to the sensor surface and the resulting frequency shift results.

Citation Download Citation

Yating Chai, Suiqiong Li, Shin Horikawa, Wen Shen, Mi-Kyung Park, Vitaly J. Vodyanoy, and Bryan A. Chin "The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor", Proc. SPIE 8027, Sensing for Agriculture and Food Quality and Safety III, 802708 (2 June 2011); https://doi.org/10.1117/12.883705

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