2 June 2011 Rapid detection of salmonella using SERS with silver nano-substrate
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Abstract
Surface Enhanced Raman Scattering (SERS) can detect the pathogen in rapid and accurate. In SERS weak Raman scattering signals are enhanced by many orders of magnitude. In this study silver metal with biopolymer was used. Silver encapsulated biopolymer polyvinyl alcohol nano-colloid was prepared and deposited on stainless steel plate. This was used as metal substrate for SERS. Salmonella typhimurium a common food pathogen was selected for this study. Salmonella typhimurium bacteria cells were prepared in different concentrations in cfu/mL. Small amount of these cells were loaded on the metal substrate individually, scanned and spectra were recorded using confocal Raman microscope. The cells were exposed to laser diode at 785 nm excitation and object 50x was used to focus the laser light on the sample. Raman shifts were obtained from 400 to 2400 cm-1. Multivariate data analysis was carried to predict the concentration of unknown sample using its spectra. Concentration prediction gave an R2 of 0.93 and standard error of prediction of 0.21. The results showed that it could be possible to find out the Salmonella cells present in a low concentration in food samples using SERS.
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J. Sundaram, J. Sundaram, B. Park, B. Park, A. Hinton, A. Hinton, W. R. Windham, W. R. Windham, S. C. Yoon, S. C. Yoon, K. C. Lawrence, K. C. Lawrence, "Rapid detection of salmonella using SERS with silver nano-substrate", Proc. SPIE 8027, Sensing for Agriculture and Food Quality and Safety III, 80270B (2 June 2011); doi: 10.1117/12.883996; https://doi.org/10.1117/12.883996
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