Development of narrow-band fluorescence index for the detection of aflatoxin contaminated corn

Haibo Yao; Zuzana Hruska; Russell Kincaid; Ambrose Ononye; Robert L. Brown; Deepak Bhatnagar; Thomas E. Cleveland

doi:10.1117/12.884400

2 June 2011 Development of narrow-band fluorescence index for the detection of aflatoxin contaminated corn

Haibo Yao, Zuzana Hruska, Russell Kincaid, Ambrose Ononye, Robert L. Brown, Deepak Bhatnagar, Thomas E. Cleveland

Proceedings Volume 8027, Sensing for Agriculture and Food Quality and Safety III; 80270D (2011) https://doi.org/10.1117/12.884400
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

Aflatoxin is produced by the fungus Aspergillus flavus when the fungus invades developing corn kernels. Because of its potent toxicity, the levels of aflatoxin are regulated by the Food and Drug Administration (FDA) in the US, allowing 20 ppb (parts per billion) limits in food, and feed intended for interstate commerce. Currently, aflatoxin detection and quantification methods are based on analytical tests. These tests require the destruction of samples, can be costly and time consuming, and often rely on less than desirable sampling techniques. Thus, the ability to detect aflatoxin in a rapid, non-invasive way is crucial to the corn industry in particular. This paper described how narrow-band fluorescence indices were developed for aflatoxin contamination detection based on single corn kernel samples. The indices were based on two bands extracted from full wavelength fluorescence hyperspectral imagery. The two band results were later applied to two large sample experiments with 25 g and 1 kg of corn per sample. The detection accuracies were 85% and 95% when 100 ppb threshold was used. Since the data acquisition period is significantly lower for several image bands than for full wavelength hyperspectral data, this study would be helpful in the development of real-time detection instrumentation for the corn industry.

Citation Download Citation

Haibo Yao, Zuzana Hruska, Russell Kincaid, Ambrose Ononye, Robert L. Brown, Deepak Bhatnagar, and Thomas E. Cleveland "Development of narrow-band fluorescence index for the detection of aflatoxin contaminated corn", Proc. SPIE 8027, Sensing for Agriculture and Food Quality and Safety III, 80270D (2 June 2011); https://doi.org/10.1117/12.884400

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available