Light without substrate amendment: the bacterial luciferase gene cassette as a mammalian bioreporter

Dan M. Close; Tingting Xu; Abby E. Smartt; Pat Jegier; Steven A. Ripp; Gary S. Sayler

doi:10.1117/12.883289

16 May 2011 Light without substrate amendment: the bacterial luciferase gene cassette as a mammalian bioreporter

Dan M. Close, Tingting Xu, Abby E. Smartt, Pat Jegier, Steven A. Ripp, Gary S. Sayler

Proceedings Volume 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII; 80290F (2011) https://doi.org/10.1117/12.883289
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

Bioluminescent production represents a facile method for bioreporter detection in mammalian tissues. The lack of endogenous bioluminescent reactions in these tissues allows for high signal to noise ratios even at low signal strength compared to fluorescent signal detection. While the luciferase enzymes commonly employed for bioluminescent detection are those from class Insecta (firefly and click beetle luciferases), these are handicapped in that they require concurrent administration of a luciferin compound to elicit a bioluminescent signal. The bacterial luciferase (lux) gene cassette offers the advantages common to other bioluminescent proteins, but is simultaneously capable of synthesizing its own luciferin substrates using endogenously available cellular compounds. The longstanding shortcoming of the lux cassette has been its recalcitrance to function in the mammalian cellular environment. This paper will present an overview of the work completed to date to overcome this limitation and provide examples of mammalian lux-based bioreporter technologies that could provide the framework for advanced, biomedically relevant real-time sensor development.

Citation Download Citation

Dan M. Close, Tingting Xu, Abby E. Smartt, Pat Jegier, Steven A. Ripp, and Gary S. Sayler "Light without substrate amendment: the bacterial luciferase gene cassette as a mammalian bioreporter", Proc. SPIE 8029, Sensing Technologies for Global Health, Military Medicine, Disaster Response, and Environmental Monitoring; and Biometric Technology for Human Identification VIII, 80290F (16 May 2011); https://doi.org/10.1117/12.883289

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available