Three-dimensional imaging of dynamic phenomena in micro-objects using phase contrast digital holographic interferometric microscopy

Arun Anand; Vani K. Chhaniwal; Bahram Javidi

doi:10.1117/12.885368

20 May 2011 Three-dimensional imaging of dynamic phenomena in micro-objects using phase contrast digital holographic interferometric microscopy

Arun Anand, Vani K. Chhaniwal, Bahram Javidi

Author Affiliations +

Proceedings Volume 8043, Three-Dimensional Imaging, Visualization, and Display 2011; 80430T (2011) https://doi.org/10.1117/12.885368
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

Imaging of transparent micro-objects objects, especially living cells, becomes a challenging problem since such objects do not appreciably change the amplitude of the electromagnetic radiation interacting with it. Phase contrast techniques can be used to overcome this hurdle. Digital holography is one of the most effective tools for phase contrast microscopic imaging of transparent objects and it yields the three dimensional profile of the object under investigation. Another advantage of digital holography is that of numerical focusing, allowing one to focus on to any desired object plane. This aspect of digital holography along with interferometry is utilized to study dynamics of phase micro-objects. The changes occurring to the object under study is determined from their three dimensional profile which is obtained from the phase information.

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Arun Anand, Vani K. Chhaniwal, and Bahram Javidi "Three-dimensional imaging of dynamic phenomena in micro-objects using phase contrast digital holographic interferometric microscopy", Proc. SPIE 8043, Three-Dimensional Imaging, Visualization, and Display 2011, 80430T (20 May 2011); https://doi.org/10.1117/12.885368

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