3 May 2011 Covalent enzyme immobilization onto carbon nanotubes using a membrane reactor
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Composite porous polysulfone-carbon nanotubes membranes were prepared by dispersing carbon nanotubes into a polysulfone solution followed by the membrane formation by phase inversion-immersion precipitation technique. The carbon nanotubes with amino groups on surface were functionalized with different enzymes (carbonic anhydrase, invertase, diastase) using cyanuric chloride as linker between enzyme and carbon nanotube. The composite membrane was used as a membrane reactor for a better dispersion of carbon nanotubes and access to reaction centers. The membrane also facilitates the transport of enzymes to active carbon nanotubes centers for functionalization (amino groups). The functionalized carbon nanotubes are isolated by dissolving the membranes after the end of reaction. Carbon nanotubes with covalent immobilized enzymes are used for biosensors fabrications. The obtained membranes were characterized by Scanning Electron Microscopy, Thermal analysis, FT-IR Spectroscopy, Nuclear Magnetic Resonance, and functionalized carbon nanotubes were characterized by FT-IR spectroscopy.
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Stefan Ioan Voicu, Stefan Ioan Voicu, Aurelia Cristina Nechifor, Aurelia Cristina Nechifor, Ovidiu Gales, Ovidiu Gales, Gheorghe Nechifor, Gheorghe Nechifor, } "Covalent enzyme immobilization onto carbon nanotubes using a membrane reactor", Proc. SPIE 8068, Bioelectronics, Biomedical, and Bioinspired Systems V; and Nanotechnology V, 80680Y (3 May 2011); doi: 10.1117/12.888780; https://doi.org/10.1117/12.888780

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