6 May 2011 An optical biosensor based on His6-OPH for organophosphate detection
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Abstract
In this work we describe a method for immobilizing the hexahistidine-modified OPH (His6-OPH) enzyme via sol-gel technology in a porous material while retaining its catalytic activity. The potential use of each bio-sensing material was checked by quantifying the enzymatic properties, such as the relative activity of the immobilized enzyme, its Michaelis- Menten equation parameters and its stability under extreme working conditions (pH, T). The bio-sensor material was also characterised by SEM as well as in terms of its reversibility and sensitivity.
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Nina Frančič, Nina Frančič, Ilya V. Lyagini, Ilya V. Lyagini, Elena N. Efremenko, Elena N. Efremenko, Aljoša Košak, Aljoša Košak, Aleksandra Lobnik, Aleksandra Lobnik, } "An optical biosensor based on His6-OPH for organophosphate detection", Proc. SPIE 8073, Optical Sensors 2011; and Photonic Crystal Fibers V, 80730T (6 May 2011); doi: 10.1117/12.886788; https://doi.org/10.1117/12.886788
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