An optical biosensor based on His6-OPH for organophosphate detection

Nina Frančič; Ilya V. Lyagini; Elena N. Efremenko; Aljoša Košak; Aleksandra Lobnik

doi:10.1117/12.886788

6 May 2011 An optical biosensor based on His₆-OPH for organophosphate detection

Nina Frančič, Ilya V. Lyagini, Elena N. Efremenko, Aljoša Košak, Aleksandra Lobnik

Proceedings Volume 8073, Optical Sensors 2011; and Photonic Crystal Fibers V; 80730T (2011) https://doi.org/10.1117/12.886788
Event: SPIE Optics + Optoelectronics, 2011, Prague, Czech Republic

Abstract

In this work we describe a method for immobilizing the hexahistidine-modified OPH (His₆-OPH) enzyme via sol-gel technology in a porous material while retaining its catalytic activity. The potential use of each bio-sensing material was checked by quantifying the enzymatic properties, such as the relative activity of the immobilized enzyme, its Michaelis- Menten equation parameters and its stability under extreme working conditions (pH, T). The bio-sensor material was also characterised by SEM as well as in terms of its reversibility and sensitivity.

Citation Download Citation

Nina Frančič, Ilya V. Lyagini, Elena N. Efremenko, Aljoša Košak, and Aleksandra Lobnik "An optical biosensor based on His₆-OPH for organophosphate detection", Proc. SPIE 8073, Optical Sensors 2011; and Photonic Crystal Fibers V, 80730T (6 May 2011); https://doi.org/10.1117/12.886788

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