8 June 2011 The multifunctional application of microfluidic lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS) within the field of bioanalytics
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Abstract
This contribution will present a variety of applications of lab-on-a-chip surface enhanced Raman spectroscopy in the field of bioanalytic. Beside the quantification and online monitoring of drugs and pharmaceuticals, determination of enzyme activity and discrimination of bacteria are successfully carried out utilizing LOC-SERS. The online-monitoring of drugs using SERS in a microfluidic device is demonstrated for nicotine. The enzyme activity of thiopurine methyltransferase (TPMT) in lysed red blood cells is determined by SERS in a lab-on-a-chip device. To analyse the activity of TPMT the metabolism of 6-mercaptopurine to 6-methylmercaptopurine is investigated. The discrimination of bacteria on strain level is carried out with different E. coli strains. For the investigations, the bacteria are busted by ultra sonic to achieve a high information output. This sample preparation provides the possibility to detect SERS spectra containing information of the bacterial cell walls as well as of the cytoplasm. This contribution demonstrates the great potential of LOC-SERS in the field of bioanalytics.
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Anne März, Anne März, Bettina Mönch, Bettina Mönch, Angela Walter, Angela Walter, Thomas Bocklitz, Thomas Bocklitz, Wilm Schumacher, Wilm Schumacher, Petra Rösch, Petra Rösch, Michael Kiehntopf, Michael Kiehntopf, Jürgen Popp, Jürgen Popp, } "The multifunctional application of microfluidic lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS) within the field of bioanalytics", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808707 (8 June 2011); doi: 10.1117/12.890086; https://doi.org/10.1117/12.890086
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