Detection of early metabolic alterations in the ocular fundus of diabetic patients by time-resolved autofluorescence of endogenous fluorophores

D. Schweitzer; M. Klemm; S. Quick; L. Deutsch; S. Jentsch; M. Hammer; J. Dawczynski; C. H. Kloos; U. A. Mueller

doi:10.1117/12.889109

10 June 2011 Detection of early metabolic alterations in the ocular fundus of diabetic patients by time-resolved autofluorescence of endogenous fluorophores

D. Schweitzer, M. Klemm, S. Quick, L. Deutsch, S. Jentsch, M. Hammer, J. Dawczynski, C. H. Kloos, U. A. Mueller

Author Affiliations +

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 80871G (2011) https://doi.org/10.1117/12.889109
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Measurements of time-resolved autofluorescence (FLIM) at the human ocular fundus of diabetic patients permit the detection of early pathologic alterations before signs of diabetic retinopathy are visible. The measurements were performed by the Jena Fluorescence Lifetime Laser Scanner Ophthalmoscope applying time-correlated single photon counting (TCSPC) in two spectral channels (K1: 490-560 nm, K2:560-700ps). The fluorescence was excited by 70 ps pulses (FWHM) at 448 nm. The decay of fluorescence intensity was triple-exponentially approximated. The frequency of amplitudes, lifetimes, and relative contributions was compared in fields of the same size and position in healthy subjects and in diabetic patients. The most sensitive parameter was the lifetime T2 in the short-wavelength channel, which corresponds to the neuronal retina. The changes in lifetime point to a loss of free NADH and an increased contribution of protein-bound NADH in the pre-stage of diabetic retinopathy.

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D. Schweitzer, M. Klemm, S. Quick, L. Deutsch, S. Jentsch, M. Hammer, J. Dawczynski, C. H. Kloos, and U. A. Mueller "Detection of early metabolic alterations in the ocular fundus of diabetic patients by time-resolved autofluorescence of endogenous fluorophores", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871G (10 June 2011); https://doi.org/10.1117/12.889109

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