10 June 2011 Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms
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Abstract
The diffusion capabilities of free fluorophores inside the heterogeneous three dimensional structure of Staphylococcus aureus biofilm were studied by an original image-based Fluorescence Recovery After Photobleaching method. The study was extended to BODIPY-vancomycin in order to better understand the mechanisms involved in the high tolerance of the bacteria embedded in a biofilm to the antibiotic.
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S. Daddi Oubekka, S. Daddi Oubekka, R. Briandet, R. Briandet, F. Waharte, F. Waharte, M.-P. Fontaine-Aupart, M.-P. Fontaine-Aupart, K. Steenkeste, K. Steenkeste, } "Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871I (10 June 2011); doi: 10.1117/12.889461; https://doi.org/10.1117/12.889461
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