14 June 2011 Testing possibilities for establishing nanodiamond-DNA-conjugates
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Abstract
We report on the biofunctionalization of nanodiamond surfaces in a two step procedure: chemical modification, resulting in homogeneous and defined surface coating, with following addition of ssDNA. Carboxylation, thymidine coupling and amination methods for chemical modification of diamond surfaces for further functionalization experiments were applied. To enable the coupling process, biomolecules were also chemically modified with functional groups (-NH2). FTIR spectroscopy, fluorescence microscopy and gel electrophoresis were applied for characterizing modified ND particles and bioconjugates and for controlling the coupling success.
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A. Pohl, A. Pohl, S. Joch, S. Joch, J. Michael, J. Michael, E. Boschke, E. Boschke, P. Quenzel, P. Quenzel, J. Schreiber, J. Schreiber, V. Lapina, V. Lapina, J. Opitz, J. Opitz, } "Testing possibilities for establishing nanodiamond-DNA-conjugates", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871T (14 June 2011); doi: 10.1117/12.889859; https://doi.org/10.1117/12.889859
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