10 June 2011 Two-channel microfluidic CARS: experimental quantification of pure vibrational contrast in CARS images
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The combination of linear and nonlinear Raman microspectroscopy has been established to be a powerful tool for biomedical diagnostics. In this contribution we discuss our recent approaches towards CARS (coherent anti-Stokes Raman scattering) based quantification of analytes, which is generally complicated by the CARS-signal strength dependence on the square of the molecular concentration and on the interplay between a molecular-specific vibrational signal and a nonresonant contribution in the signal generation. Due to these complications the quantification of analytes presents a major challenge in CARS microscopy. Here we discuss two recently developed approaches, i.e. on the one hand a simplified setup for coherent anti-Stokes Raman scattering (CARS) microscopy, which allows for recording CARS images with 30 cm-1 excitation bandwidth for probing Raman bands between 500 and 900 cm-1 with minimal requirements for alignment. This experimental arrangement is based on electronic switching between CARS images recorded at different Raman resonances by combining a photonic crystal fiber (PCF) as broad-band light source and an acoustooptical programmable dispersive filter (AOPDF) as tunable wavelength filter. On the other hand, we discuss how the introduction of carbon-deuterium (C-D) bonds into drug compounds constitutes a non-invasive labeling approach that allows for higher intrinsic CARS contrast to be obtained. The quantitative detection of C-deuterated drugs by Raman microspectroscopy and CARS microscopy is examined. Concentration-dependent studies on drugs with aliphatic and aromatic C-D moieties were performed in a two-channel microfluidic chip, using the corresponding non-deuterated (C-H) isotopomers as an internal reference.
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G. Bergner, G. Bergner, T. Henkel, T. Henkel, D. Akimov, D. Akimov, B. Dietzek, B. Dietzek, S. Schlücker, S. Schlücker, H. Bartelt, H. Bartelt, J. Popp, J. Popp, } "Two-channel microfluidic CARS: experimental quantification of pure vibrational contrast in CARS images", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871U (10 June 2011); doi: 10.1117/12.889925; https://doi.org/10.1117/12.889925

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