Comparison of temporal response to cerebral blood flow measured by laser speckle flowgraphy and laser Doppler flowmetry

Masashi Kusano; Haruka Nakayama; Hiroyuki Takuwa; Kazuto Masamoto; Iwao Kanno; Eiji Okada

doi:10.1117/12.889787

14 June 2011 Comparison of temporal response to cerebral blood flow measured by laser speckle flowgraphy and laser Doppler flowmetry

Masashi Kusano, Haruka Nakayama, Hiroyuki Takuwa, Kazuto Masamoto, Iwao Kanno, Eiji Okada

Author Affiliations +

Proceedings Volume 8088, Diffuse Optical Imaging III; 80880E (2011) https://doi.org/10.1117/12.889787
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Cerebral blood flow (CBF) change of mice during whisker stimulation is measured by laser speckle flowgraphy (LSFG) and laser Doppler flowmetry (LDF). Laser speckle flowgraphy (LSFG) has been used to obtain the two-dimensional distribution of the blood flow in tissue as well as scanning laser Doppler flowmetry (LDF). There are several parameters of LSFG to obtain the blood flow maps and the distribution change of blood flow. In this study, we calculate four parameters from the speckle pattern to measure CBF in awake mice. The temporal resolution of LSFG is likely to be less than that of LDF. The temporal changes in CBF obtained from the four parameters calculated from the speckle pattern detected by a common CCD camera are compared with those measured by LDF. The time courses of CBF change measured by LSFG highly correlate with those by LDF. The results indicate that the temporal response of LSFG is sufficient to measure CBF change evoked by brain activations.

Citation Download Citation

Masashi Kusano, Haruka Nakayama, Hiroyuki Takuwa, Kazuto Masamoto, Iwao Kanno, and Eiji Okada "Comparison of temporal response to cerebral blood flow measured by laser speckle flowgraphy and laser Doppler flowmetry", Proc. SPIE 8088, Diffuse Optical Imaging III, 80880E (14 June 2011); https://doi.org/10.1117/12.889787

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